By Henry Y. Wang and Tadayuki Imanaka (Eds.)
content material: creation of engineered antibodies in myeloma and hybridoma cells : improvements in gene expression and approach layout / D.K. Robinson, D. DiStefano, S.L. Gould, G. Cuca, T.C. Seamans, D. Benincasa, S. Munshi, C.P. Chan, J. Stafford-Hollis, G.F. Hollis, D. Jain, okay. Ramasubramanyan, G.E. Mark, and M. Siberklang --
Antibody construction in human hybridomas with growth-associated creation kinetics / Hidekazu Sawada and Kazuaki Kitano --
Antibody creation in chinese language hamster ovary cells utilizing an impaired selectable marker / R.S. Barnett, K.L. Limoli, T.B. Huynh, E.A. Ople, and M.E. Reff --
Antibody construction in insect cells / Kathleen N. Potter, Yucheng Li, and J. Donald Capra --
Antibody expression in crops / J. K-C. Ma --
Engineering of immunoglobulin Fc and single-chain Fv proteins in Escherichia coli / David Filpula, Michele Rollence, Nina Essig, James Nagle, Aniruddha Achari, and Timothy Lee --
T-cell detailed immunofusion proteins from Escherichia coli : intent, layout, and houses / Marc larger and Patricia A. Nolan --
construction and purification of antibody utilizing Bacillus subtilis as an expression host / S.L. Wong, X.C. Wu, L.P. Yang, S.C. Ng, and N. Hudson --
Trichoderma reesei, a promising novel host for antibody creation / Eini Nyyssönen and Sirkka Keränen --
utilizing antibody catalysis to natural synthesis / Brian J. Lavey and Kim D. Janda --
powerful and particular peroxidase task with an antibody L chain-porphyrin Fe(III) advanced / Tadayuki Imanaka and Masahiro Takagi.
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Selectivity of the vectors has been achieved by intentional impairment of the NEO gene by two different mechanisms. The NEO translation initiation site has been impaired and an intron has been introduced into the gene. Selection in G418 for clones containing the NEO gene yields an increased percentage of high expression clones from a decreased number of total clones. The majority of high expressing clones contain a single gene copy. Subsequent gene amplification results in clones producing very high levels of immunoglobulin with only a few gene copies.
Gehring, W. J. Annu Rev Cell Biol 1990, 6, 679714. ; ACS Symposium Series; American Chemical Society: Washington, DC, 1995. ch003 (7) (8) (9) (10) (11) (12) (13) (14) (15) (16) ANTIBODY EXPRESSION AND ENGINEERING Conklin, K. ; Groudine, M . Molecular and Cellular Biology 1986, 6, 3999-4007. ; zur Hausen, H. Proc Natl Acad Sci 1991, 88, 1411-1415. ; Bishop, J. O. Molecular and Cellular Biology 1990, 10, 1192-1198. Yoshimura, F. ; Chaffin, K. Molecular and Cellular Biology 1987, 7, 1296-1299. Reff, M .
Cytotechnology, 6, 197-208. ; ACS Symposium Series; American Chemical Society: Washington, DC, 1995. Chapter 3 Antibody Production in Chinese Hamster Ovary Cells Using an Impaired Selectable Marker R. S. Barnett, K. L. Limoli, T. B. Huynh, E. A. Ople, and M. E. ch003 Department of Gene Expression, IDEC Pharmaceuticals Corporation, 11011 Torreyana Road, San Diego, CA 92121 The expression levels of transfected genes in mammalian cells are primarily determined by the cellular DNA at the site of integration.