By A. D. Curson (auth.), D. A. Hemsley (eds.)
Synthetic polymers make very good specimens for gentle microscopy. regardless of this, using the procedure, no less than in its complicated kinds, isn't really so frequent as can be anticipated. even if trustworthy and suitable info are tricky to discover and quantify, it sounds as if in different fields of fabrics technological know-how and know-how there's a larger readiness to tum to the microscope in examine, in business challenge fixing, or for caliber overview and keep watch over. It additionally turns out that the explanations for the current scenario are partially historic, partially the results of the constitution of the plastics and rubber industries, and partially the schooling and coaching historical past of senior employees who are typically chemistry or engineering dependent. In neither box does gentle microscopy function strongly within the uncomplicated education. the first objective of this ebook is to supply a few perception into the diversity oflight microscopy suggestions appropriate to polymeric specimens, and to spotlight general purposes to advertisement polymers and polymer items. the place acceptable, the optical innovations concerned are mentioned in a few aspect. despite the fact that, it has no longer been the goal to supply a gentle microscopy textbook facing the rules and layout of the elemental tool. Many such texts can be found, and chosen examples are brought up within the reference record on the finish of such a lot chapters.
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12); it also helps to prevent curling of the section. However, extreme care should be taken when using either aid. Pulling with forceps tends to Specimen Preparation 29 FIG. 12. Removing a thin section with the help of a soft-haired brush. damage and stretch the section, and heavy handedness with even a soft brush on polyethylene will leave severe brush marks on the section. Applying a small amount of the mounting liquid, water or alcohol via a pipette, spray or bath to the knife edge during cutting will reduce 'judder' and make retrieval of the section easier.
The sections need to be allowed to dry before being mounted. One must ensure, having finished using a cold stage on a microtome, that the equipment is thoroughly dried and oiled to prevent the formation of rust. Specimen Preparation 27 Fibres Fibres can be treated in much the same way as powders, with the fibres supported parallel to the longitudinal axis of the moulding capsule. On occasions it may be necessary to section fibres parallel to their long axis; this can be done by embedding the fibres in a sheet of acrylic.
The coverslip should be supported in the vertical position to the work table with a small amount of wax. When the wax and the balsam have hardened, the blade can be advanced and the second cut made (see Fig. 17). The wax support can be removed carefully with xylene, and the section left on the coverslip with a slice being mounted on the second surface. Alternatively the section can be removed using xylene and mounted in the normal way. Saw Blade Section Being Cut Cover Slip Natural Canada Balsam ---1+-+-- - - - Specimen FIG.