By Kathy Barker
A guide for dwelling and dealing within the laboratory. It discusses: how learn teams paintings on a human point - and the way to slot in; what gear is key, and the way to exploit it thoroughly; easy methods to start and get equipped; tips on how to manage an test; tips to deal with and use info and reference assets; and the way to give your self and your effects - in print and in individual. It deals recommendation, ethical aid, social etiquette, coverage, besides step by step directions for these simple laboratory approaches that skilled investigators comprehend - yet beginners don't.
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I got by with a lot of help from my friends, who, like most people in cytometry, are almost always eager to help other people learn about what they’re doing and how to do it. At meetings, and via email, I asked a whole bunch of people to send me their good stuff and to put me in touch with other people who were doing interesting things in their facilities. I did the same thing, on a much more limited basis, when I wrote the Third Edition, but the response this time around was much more dramatic; this edition is really a cooperative venture.
This time around, I’ve tried to explain what flow cytometry is and flow cytometers are first, then consider how they got to be that way, and get into the real details after that. I’ve retained a lot of the practices I adopted in the previous editions. When I want a word or phrase to catch your attention, I’ve put it in boldface. I still emphasize the fact that flow cytometry rests on the same foundations as other techniques of analytical cytology; the optics and spectroscopy are the same, as are many of the parameters measured and the probes used for their measurement.
However, whether you are a flow novice or an old hand, you are likely to benefit from skimming the book from cover to cover. You may find things you wouldn’t have found just using the Table of Contents and the Index; these are likely to be the most idiosyncratic parts of any book, and this book is more idiosyncratic than most. ACKNOWLEDGEMENTS Leslie Hochberg Shapiro continues to keep me in love and at large, with additional legal help from Peter. Jill and Mike offered moral support when they weren’t busy taking care of patients and/or kids, and such kids (they have added “There’s No Business Like Flow Business” [p.