By Frank H. Stephenson
Calculations in Molecular Biology and Biotechnology: A consultant to arithmetic within the Laboratory is the 1st finished consultant committed solely to calculations encountered within the genetic engineering laboratory. arithmetic, as an essential element of the profitable layout and interpretation of simple learn, is used day-by-day in laboratory paintings. This advisor, written for college kids, technicians, and scientists, presents instance calculations for the main usually faced difficulties encountered in gene discovery and research. The textual content and pattern calculations are written in an easy-to-follow structure. it's the excellent laboratory better half for an individual operating in DNA manipulation and research. *A entire consultant to calculations for a large choice of difficulties encountered within the simple study laboratory. * instance calculations are labored via from begin to end in easy-to-follow steps * Key chapters dedicated to calculations encountered while operating with micro organism, phage, PCR, radioisotopes, recombinant DNA, centrifugation, oligonucleotides, protein, and forensic technological know-how. *Written for college students and laboratory technicians yet an invaluable reference for the more matured researcher. *A worthwhile instructing source.
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Extra info for Calculations for Molecular Biology and Biotechnology: A Guide to Mathematics in the Laboratory
When the sodium chloride is completely dissolved, dispense the solution into a 50-mL graduated cylinder and bring the final volume up to 47 mL with distilled water. 95 x 200 = 190 Therefore, to prepare 200 mL of 95% ethanol, measure 190 mL of 100% (200 proof) ethanol and add 10 mL of distilled water to bring the final volume to 200 mL. Diluting Percent Solutions When approaching a dilution problem involving percentages, express the percent solutions as fractions of 100. The problem can be written as an equation in which the concentration of the stock solution ("what you have") is positioned on the left side of the equation and the desired final concentration ("what you want") is on the right side of the equation.
5 M sodium carbonate solution. pH The first chemical formula most of us learn, usually during childhood, is that for water, I-I20. A water molecule is composed of two atoms of hydrogen and one atom of oxygen. The atoms of water, however, are only transiently associated in this form. At any particular moment, a certain number of water molecules will be dissociated into hydrogen (H § and hydroxyl (OH-) ions These ions will reassociate within a very short time to form the I-/20 water molecule again.
1 mL of the dilution series to be made will be plated. 1 x 10 -7 m L , the following serial dilution can be performed. 1 mL 10 mL 10 mL 10 mL Note: There is no one correct way to determine a dilution series that will achieve the dilution you want. The volumes you use for the dilutions are dictated by several parameters, including the amount of dilution buffer available, the accuracy o f the pipets, and the size of the available dilution tubes. Plotting ODsso vs. 1. When cells from an overnight culture are inoculated into fresh medium, as described in this chapter, they experience a period during which they adjust to the new environment.