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Yet, when the same experiments are performed with ionophore treated membranes, the rate of vanadate dissociation increases much more steeply. 5 mM which indicates that vanadate dissociation is initiated by the occupation of the low affinity calcium binding sites. The fact, that the rate of vanadate dissociation is very much accelerated by the ionophore shows that calcium has to reach the interior of the vesicles in order to expel vanadate from the enzyme. 44 Wilhelm Hasselbach et al. 1 Ca44 Concentration ( mM ) 2 FIGURE 12.
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