Download Cystic Fibrosis Methods and Protocols (Methods in Molecular by William R. Skach PDF

By William R. Skach

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Et al. (1998) Genomic DNA sequence of Rhesus (M. mulatta) cystic fibrosis (CFTR) gene. Mamm. Genome. 9, 301–305. 43. , Riordan, J. , et al. (1991) Genomic DNA sequence of the cystic fibrosis transmembrane conductance regulator (CFTR) gene. Genomics 10, 214–228. 44. , and Sekiya, T. (1989) Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms. Proc. Natl. Acad. Sci. USA 86, 2766–2770. 45. , and Dean, M. (1994) Sensitivity of single-strand conformation polymorphism and heteroduplex method for mutation detection in the cystic fibrosis gene.

Genet. 6, 2099–2107. 40. Snouwaert, J. , Brigman, K. , Latour, A. , Malouf, N. , Boucher, R. , and Koller, B. H. (1992) An animal model for cystic fibrosis made by gene targeting. Science 257, 1083–1088. 41. Wine, J. , and Moss, R. B. (2001) Comprehensive mutation screening in a cystic fibrosis center. Pediatrics 107, 280–286. 42. Wine, J. , et al. (1998) Genomic DNA sequence of Rhesus (M. mulatta) cystic fibrosis (CFTR) gene. Mamm. Genome. 9, 301–305. 43. , Riordan, J. , et al. (1991) Genomic DNA sequence of the cystic fibrosis transmembrane conductance regulator (CFTR) gene.

Alternatively, calculate spectra on-line when currents are in a steady state. 5. 2 Hz to fmax = 330 Hz; see Note 7). Average at least 10 spectra calculated from consecutive segments to smooth the data sufficiently. Fifty seconds of steady-state current are needed (see Note 8). 2. Analysis of Activating or Inactivating (Nonstationary) Currents This analysis allows the calculation of N and Po of multichannel recordings. An example of this analysis is shown in Fig. 5. 1. Visually inspect the current and noise signals.

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