Download Endogenous Interferences in Clinical Laboratory Tests by Martin H. Kroll PDF

By Martin H. Kroll

Scientific laboratories needs to supply exact attempt effects to guard sufferer safeguard. medical laboratory samples usually comprise excessive quantities of bilirubin or lipemia. This publication offers the empirical and theoretical beginning for bilirubinemia or lipemia and the impression they've got at the caliber of effects and sufferer security. It discusses the origins of interferences and their right review.

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Sample text

Dyes possess several resonance structures [7]. In molecular orbital theory, there are several types of orbitals involved, including bonding or antibonding orbitals of sigma (σ or σ*) or pi (π or π*) types, and in addition, n-type nonbonding orbitals [7]. In the lowest energy state of the molecule, all of the bonding and nonbonding orbitals are fully occupied, while the antibonding orbitals are empty [7]. , an electron transfers from a nonbonding or bonding orbital to an antibonding orbital, usually n → π* and π → π* [7].

This interfering potential is an important problem for co-oximetry, where hemoglobin in its various forms is measured without chemical alteration. One of the most common ways to minimize interferences with methods is using a sample blank. In a manual spectrophotometer, that process is rather easy, because one would use a sample diluted with buffer as the blank in the analyzer. Automated equipment used to present a challenge, because automated analyzers used a single blank channel for multiple samples.

Ann Clin Biochem UK 1987,24,85–97. Soldin SJ, Henderson L, Hill JG. The effect of bilirubin and ketones on reaction rate methods for the measurement of creatinine. Clin Biochem USA 1978,11,82–86. 5 References 33 [18] da Fonseca-Wollheim F, Heinze K-G, Lomsky K, Schreiner H. Serum ultrafiltration for the elimination of endogenous interfering substances in creatinine determination. J Clin Chem Clin Biochem Germany 1988,26,523–525. [19] Schoenmakers CH, Kuller T, Lindemans J, Blijenberg BG. Automated enzymatic methods for creatinine measurement with special attention to bilirubin interference.

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