Download Forensic DNA Profiling Protocols (Methods in Molecular by Patrick J. Lincoln PDF

By Patrick J. Lincoln

Royal institution of drugs and Dentistry, London, united kingdom. cutting-edge advances in id trying out via DNA research. Covers PCR-based try out structures, direct-phase minisequencing, and extra. DNLM: DNA Fingerprinting--methods--laboratory manuals.

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Forensic DNA Profiling Protocols (Methods in Molecular Biology)

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3. 5 EDTA-Na . Store at 4. 0, 1 mM 2 4. Water-saturated n-butanol. Prepare fresh before use. 5. , Danvers, MA). 6. 0+ Human DNA Quantitation System (Gibco-BRL, Gaithersburg, MD). 3— PCR and Detection of PCR Products 1. , Multiplex PCR kits: AmpliType PM PCR Amplification and Typing Kit [Roche]; GenePrint STR Systems PCR Amplification Kit [Promega Corporation, Madison, WI]). 2. SA 32 Electrophoresis Apparatus (Gibco-BRL). 4 mm. 3. 5% acetic acid in 95% ethanol) to prepare glass plates. 4. 5X Trisborate-EDTA buffer) (210 g urea, 267 mL dH O, 25 mL 10X TBE, and 50 mL 40% acrylamide:bis [19:1]).

Water-saturated n-butanol. Prepare fresh before use. 5. , Danvers, MA). 6. 0+ Human DNA Quantitation System (Gibco-BRL, Gaithersburg, MD). 3— PCR and Detection of PCR Products 1. , Multiplex PCR kits: AmpliType PM PCR Amplification and Typing Kit [Roche]; GenePrint STR Systems PCR Amplification Kit [Promega Corporation, Madison, WI]). 2. SA 32 Electrophoresis Apparatus (Gibco-BRL). 4 mm. 3. 5% acetic acid in 95% ethanol) to prepare glass plates. 4. 5X Trisborate-EDTA buffer) (210 g urea, 267 mL dH O, 25 mL 10X TBE, and 50 mL 40% acrylamide:bis [19:1]).

Centrifuge samples for 3 min at 10,000g to pellet the Chelex 100 resin, substrate, and remaining tissue/bone. 13. Extracts are now ready for quantitation and the PCR amplification process see ( Note11 ). 14. For short-term storage (up to 1 mo), store extracts at 2  on the Chelex 100 resin. To use the extracts after storage, repeatsteps 11 and 12. For long-term storage (>1 mo), the extracts should be centrifuged and the supernatant removed from the Chelex 100 resin and stored in a new tube at -20 (see Note12 ).

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