Download Genome visualization by classic methods in light microscopy by Jean-Marie Exbrayat PDF

By Jean-Marie Exbrayat

"Genome Visualization by means of vintage equipment in mild Microscopy covers the vintage ideas used to imagine nucleic acids with using mild microscopy. The ebook discusses the chemical constitution of nucleic acids, normal rules of staining, nuclear dyes, and histochemical equipment. additionally, the booklet addresses concerns relating frozen sections, smears and quantitative research. every one bankruptcy positive aspects the Read more...

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5 g. 10 mL 10 mL Physical Fixation In addition to chemical fixation by means of molecules that react with the tissue component, there are methods that are based exclusively on physical principles. 1 Cryodesiccation In cryodesiccation a fresh tissue is frozen quickly, then dried under vacuum at a very low temperature. Tissue water goes directly from the solid state to the gaseous state. The tissue fragment is then directly embedded in melted wax. ➫Cryodesiccation has a lyophilization phase that avoids tissue component diffusion.

2 ➫This method is especially useful for insects, small crustaceans, and lungs. Protocol ❑ Dehydration ● Pieces preserved by Bouin’s fluid or formaline 1. Ethanol 70% 4h 2. Ethanol 96% 2 × 12 h 3. Ethanol 100% 2×4h 4. Acetone (propanone) 2 × 30 min ● Organs that are preserved in Carnoy’s fluid are directly immersed in butanol. ❑ Celloidin impregnation 5. Add methyl salicylate until pieces fall to the bottom of the receptacle. 37 Tissue Preparation 6. Add celloidin 1% in methyl salicylate until pieces fall to the bottom of the receptacle.

1 Definitions . . . . . . . . . . . . . . . . . . . . . . . 1 Smears . . . . . . . . . . . . . . . . . . . . . . 2 Imprint . . . . . . . . . . . . . . . . . . . . . . 3 Squash . . . . . . . . . . . . . . . . . . . . . . 2 Making a Smear . . . . . . . . . . . . . . . . . . . . . 1 Dry Blood Smear on Slide . . . . . . . . . . . . . . . 2 Dry Blood Smear on Lamella .

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