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Lampen and Peterson suggest that part of the bound PABA in natural materials is in a form which resists acid hydrolysis. On the other hand, Pennington 5 reports that tissue PABA, but not pure PABA, is virtually completely destroyed by alkaline hydrolysis. The first indication that not all of the PABA of yeast is in the free state was the report by Loomis and his associates9 of an antisulfanilamide fraction from yeast which was ether-insoluble, not diazotizable, and not inactivated by acetylation.

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Shive, W. W. Ackermann, M. Gordon, M. E . Getzendaner, and R. E . E a k i n , / . Am. Chem. Soc. 69, 725 (1947). 10 M. R. Stetten and C. L. Fox, J r . , / . Biol. Chem. 161, 333 (1945). 11 M. G. Sevag and M. N . Green, / . Bacteriol. 48, 615 (1944). 12 J. Tabone and C. Magis, Bull. soc. chim. biol. 28, 744 (1946). 13 H . W. Eckert,

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